FastQCFastQC Report
Thu 23 Jun 2016
P1234_1020_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP1234_1020_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15843059
Sequences flagged as poor quality0
Sequence length123
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA2996971.891661200024566No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC1181040.7454620979445952No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT433140.27339417217344203No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA199850.1261435686126019No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC179530.11331776268711743No Hit
GAGCACACGTCTGAACTCCAGTCACTCCGCGAAATCTCGTATGCCGTCTT160910.10156498186366661TruSeq Adapter, Index 6 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG437500.0112.108691
TCTACGG449050.0108.756822
CTACGGC459600.0105.9934163
TACGGCC455800.0105.762044
CGGCCAT461150.0104.19786
GCCATAC468550.0101.738288
ACGGCCA475550.0101.134985
GGCCATA492450.097.7404567
CCATACC489700.096.974469
TTCACGA42100.072.01623
GTTCACG41150.071.975232
TCACGAC41950.071.438174
CTTAGCG293100.059.509466
TTAGCGG296650.058.836327
AGCGGTG298600.057.8831449
CGACTCT309600.057.191291
TAGCGGT306350.056.7048238
GTGCTCG22650.053.3884161
TCTTAGC327900.053.3217935
CACGACG100150.052.9638821