FastQCFastQC Report
Thu 23 Jun 2016
P1234_1008_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameP1234_1008_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16710354
Sequences flagged as poor quality0
Sequence length123
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAA4462292.6703743080487703No Hit
CGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGC3673612.198403456922576No Hit
AGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAGCGGAGGA1169200.6996859551868261No Hit
GACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCT1003810.6007113912727402No Hit
ATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGG677570.4054791418542061No Hit
AACGCGACCGATCCCGGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCT586050.35071070307666735No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC503850.3015196446466664No Hit
CTCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAA420170.2514429077923783No Hit
AGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGAT417680.24995281368665198No Hit
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGATCGGAAGAGCACACGT401250.24012058631432942No Hit
ACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTAAGCAGGGTCGG340030.2034846179799662No Hit
GTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC316180.18921202985885277No Hit
GCCGGGCGCGGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAG293890.1758729946714474No Hit
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGATCGGAAGAGCACACGTCT290630.1739221084125447Illumina Multiplexing PCR Primer 2.01 (100% over 21bp)
AAGGCGAGCTCAGGGAGGACAGAAACCTCCCGTGGAGCAGAAGGGCAAAA289700.17336556724052646No Hit
ACGGCCATACCACCCTGAACGCGCCCGATCTCGTCTGATCTCGGAAGCTA285140.17063672020353368No Hit
CGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACG275040.16459256338914185No Hit
GTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGAAGTGTCGATGA264900.15852446932003955No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA256320.15338992818464528No Hit
ACCGATTGGATGGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGG246280.14738167725231913No Hit
ACGGACAGGATTGACAGATTGATAGCTCTTTCTCGATTCCGTGGGTGGTG241230.1443595988451232No Hit
CGGCGGGGGACCGTCCCCCGACCGGCGACCGGCCGCCGCCGGGCGCATTT240740.14406636747492002No Hit
AACCCGGCGGCGGGTCCGGCCGTGTCGGCGGCCCGGCGGATCTTTCCCGC240630.14400054002446627No Hit
ACGATCCTTCTGACCTTTTGGGTTTTAAGCAGGAGGTGTCAGAAAAGTTA233440.13969781849026058No Hit
GGAGAAGCCGGCGGGAGCCCCGGGGAGAGTTCTCTTTTCTTTGTGAAGGG221350.13246278325402322No Hit
GGGGAGAGTTCTCTTTTCTTTGTGAAGGGCAGATCGGAAGAGCACACGTC219090.13111032836288208No Hit
ACCACTACTCTGATCGTTTTTTCACTGACCCGGTGAGGCGGGGGGGCGAG211920.12681957545603162No Hit
ACTGACCCGGTGAGGCGGGGGGGCGAGCCCCGAGGGGCTCTCGCTTCTGG204950.1226485088227335No Hit
GGATCGGCCCCGCCGGGGTCGGCCCACGGCCCTGGCGGAGCGCTGAGAAG198520.11880059512802661No Hit
AAGGGAAAGTTGAAAAGAACTTTGAAGAGAGAGTTCAAGAGGGCGTGAAA193160.11559300299682461No Hit
AGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCG189550.11343266575920535No Hit
ATCAGACCCCAGAAAAGGTGTTGGTTGATATAGACAGCAGGACGGTGGCC180610.10808268933141692No Hit
TCGTACGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAAC177680.10632928542387553No Hit
ACCGCACGTTCGTGGGGAACCTGGCGCTAAACCATTCGTAGACGACCTGC174520.10443824230174897No Hit
GGTTTAGTGAGGCCCTCGGATCGGCCCCGCCGGGGTCGGCCCACGGCCCT169660.10152986585442775No Hit
AGTGAAACTGCGAATGGCTCATTAAATCAGTTATGGTTCCTTTGGTCGCT167200.10005772468973427No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG611000.0110.573051
TCTACGG613600.0109.809392
CTACGGC617900.0108.979033
TACGGCC617500.0108.680684
CGGCCAT658700.0101.521246
GCCATAC666850.099.67138
ACGGCCA681850.098.182515
GGCCATA685700.097.608667
CCATACC695300.095.492189
CGACCGA202550.086.349115
AACGCGA208150.084.981481
ACCGATC205700.084.908387
TTCACGA32850.082.869633
CCGATCC213450.081.712378
GAAAGTT344150.081.1538855
GGAAAGT349100.080.573334
CGATCCC217400.079.824699
AAGTTGA358400.078.395437
AAAGTTG361400.077.599436
GTTCACG34650.077.5531542